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  • May 31, 2021

The Rat Brain-Derived Neurotrophic Factor (Rt BDNF) ELISA quantitates Rt BDNF in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt BDNF.

BDNF rat sandwich solid-phase ELISA (Enzyme-Linked Immunosorbent Assay) was developed to measure the number of targets bound between a matched antibody pair. The specific target antibody is pre-coated in the provided microtiter plate holes. There are many companies that also provide Human BDNF ELISA Kit online.

The sample, standard, or control is then added to these wells and bound to immobile antibodies. The sandwich is formed by adding a second antibody (detector), adding a substrate solution that reacts with the enzyme-antibody target complex to produce a measurable signal.

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The intensity of this signal is directly proportional to the target concentration present in the original sample. Each batch produced from this ELISA kit is quality checked based on criteria such as sensitivity, specificity, precision, and consistency between batches. More information on validation can be found in the manual.

BDNF (a neurotrophic factor that comes from the brain) belongs to the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing yields a mature 14 kDa protein, pro-BDNF is biologically active and secreted into synaptic vesicles along with the mature form.

BDNF is widespread in the central nervous system and has autocrine and paracrine effects on several classes of neurons. Signal transmission is mainly mediated by the tyrosine kinase receptor TrkB, although binding to a lower affinity receptor, p75-NTR, has also been demonstrated.

BDNF promotes neuronal survival and differentiation and has been shown to play an important role in memory formation and synaptic regulation. BDNF is induced by cortical neurons and is required for the survival of striatal neurons in the brain.

George Brooks

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